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Nature Genetics, January 2009, Volume 41, Number 1

Common variants in the NLRP3 region contribute to Crohn's disease susceptibility

Alexandra-Chloe Villani, Mathieu Lemire, Genevieve Fortin, Edouard Louis, Mark S Silverberg, Catherine Collette, Nobuyasu Baba, Cecile Libioulle, Jacques Belaiche, Alain Bitton, Daniel Gaudet, Albert Cohen, Diane Langelier, Paul R Fortin, Joan E Wither, Marika Sarfati, Paul Rutgeerts, John D Rioux, Severine Vermeire, Thomas J Hudson & Denis Franchimont

abstract

We used a candidate gene approach to identify a set of SNPs, located in a predicted regulatory region on chromosome 1q44 downstream of NLRP3 (previously known as CIAS1 and NALP3) that are associated with Crohn’s disease. The associations were consistently replicated in four sample sets from individuals of European descent. In the combined analysis of all samples (710 father-mother-child trios, 239 cases and 107 controls), these SNPs were strongly associated with risk of Crohn’s disease (Pcombined ¼ 3.49 109, odds ratio ¼ 1.78, confidence interval ¼ 1.47–2.16 for rs10733113), reaching a level consistent with the stringent significance thresholds imposed by whole-genome association studies.

In addition, we observed significant associations between SNPs in the associated regions and NLRP3 expression and IL-1b production. Mutations in NLRP3 are known to be responsible for three rare autoinflammatory disorders1,2. These results suggest that the NLRP3 region is also implicated in the susceptibility of more common inflammatory diseases such as Crohn’s disease.

LIST OF PARTICIPANTS

Legend: Coordinating PI Participants

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AZ Groeninge	x	x					x				x
AZ Maria Middelares		x									x
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AZ Sint-Lucas Gent							x
AZ Turnhout campus Sint-Jozef							x
CHC Clinique Saint-Joseph - Liège	x			x			x
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CHU UCL Namur site Godinne		x	x			x			x		x		x
Clinique Saint-Jean							x
Clinique Saint-Pierre	x						x						x
Hôpital de Joliment			x	x					x	x
Hôpital Erasme	x	x		x	x	x	x	x			x		x
Mariaziekenhuis Noord-Limburg				x			x
Onze Lieve Vrouwziekenhuis campus Aalst	x	x					x				x
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UZ Brussel												x
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UZ Leuven Gasthuisberg	x	x		x		x	x			x	x		x
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- -			x	x					x	x
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Thomas Billiet
Patrick Bontems
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Katrien Bulté
Guillaume Burnet
Peter Burvenich
Julie Busschaert
Philippe Caenepeel
Olivier Cajot
Christophe Claessens							x
Jean-Charles Coche
Jean-Louis Coenegrachts
Arnaud Colard							x
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Anneline Cremer
Anneline Cremer						x		x
Cléo Croonen
Francois D'Heygere	x
Steven De Coninck
Elisabeth De Greef												x
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Marc De Reuck
Elodie De Ruyck
Nicolas de Suray
Martine De Vos				x		x	x						x
Benedicte De Vroey
Astrid De Zutter
Stefan Delen
Marie-Armelle Denis
Pieter Dewint		x									x
Olivier Dewit	x						x						x
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Joris Dutre
Anke Engelen
Marc Etienne
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Aranzazu Jauregui Amezaga
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Pierre Lammens
Katrien Lecluyse
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Triana Lobaton
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Marie-Christine Mairlot
Jean-Marc Maisin
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Ludwig Marchal
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Fady Mokaddem
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Maja Noman
Hanne Ooms
An Outtier
Romy Ouziel
Harald Peeters
Annelies Posen
Philippe Potvin
Lieven Pouillon
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João Sabino
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Alexandra Sermeus
Francoise Smets
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Dirk Staessen
Marjan Steppe
Beatrijs Strubbe							x
Jo Swinnen
Clara Thienpont
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Haydeh Vafa
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Evi Van Dyck
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Catherine Van Kemseke
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Liv Vandermeulen
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Veroniek Vandewalle
Sarah Vervloesem
Valerie Wambacq
Natascha Wouters

Scientific papers

Common variants in the NLRP3 region contribute to Crohn's disease susceptibility

LIST OF PARTICIPANTS